With the advances in Next Generation Sequencing technologies, the NGS data analysis has become very important and critical task in any lab working in Genomics. Analyzing Big Genomics data (like NGS data) needs very sophisticated tools and computational infrastructure. The tools can be easily made available as most of the tools are open source, but the computation power and expertise needed to use these tools and perform NGS data analysis can become a challenging task. We have designed this online workshop to understand and learn NGS data analysis using command line tools. In this workshop we will focus on Clinical Variant Analysis widely used in Precision Genomics. The workshop will cover various aspects of NGS data analysis that can also be applied to other DNA sequencing approaches.